Tests to evaluate the Anti-virus Effect of Polymer OCT 57

Virus used: Yeast virus

Process of test

A. Prepared 6 actively growing aliquots of Yeast in a culture medium of: Sucrose, flour and water each culture 80 ml. in volume- maintained at 26-30 C.

B. Aliquot 6 was set aside as a control.

C. Aliquot 3 was milled in a rotary glass mill with 80 ml. of polished and washed beach pebbles, 0.1 gr. Spalerite, 0.1 gr. Manganite, 0.05 gr. Cobalt Oxide

The milling continued 12 hours. The dispersed liquid resulting (presumably containing the Virus and any Zipoids) was divided into two parts-

The parts were designated TV1 and TV2.

D. To determine the Virus/Zipoid content of TV1 it was mixed with Aliquot 1 and held for 10 hours at 30 C.

The resultant product (aliquot 1 plus ½ of aliquot 3 processed to TV1) was compared to 50 ml. of aliquot 6. Aliquot 6 was a normal growing culture – microscopic examination showed normal dividing cells.

Aliquot 1 plus ½ <aliquot 3 processed to TV1> was a clear liquid over a grayish layer of cloud-

microscopic

examination showed various powder like debris with no cells

in any part of the liquid or sediment .

Since the cells of Aliquot 1 had been destroyed by the <presumed> Virus/Zipoid of TV1

 

 

 

 

 

76

 

 

 

TV 1 WAS REPORTED - - - - - - - - - VIRUS/ZIPOID PRESENT

 

E. In order to evaluate the propagation capability of the Virus/Zipoid now having been shown present in Aliquot 1plus TV1. Aliquot 1 plus TV1 was mixed with the normally growing Aliquot 2 and held at 26-30 C. 10 hours. 50 ml. of the product was compared to 50 ml. of Aliquot 6.

Aliquot 6 was normally growing culture – microscopic examination showed normal dividing cells.

Aliquot 2 mixture with the Virus/Zipoid from <Aliquot 1 plus ½ Aliquot 3 processed> was slightly turbid liquid over a fluffy sediment. Microscopic examination showed cellular debris but no cells.

Since the cells of Aliquot 2 mixture had been destroyed by a substance propagating through Aliquot 1, the propagating substance was identified as a Virus rather than a Zipoid which does not destructively propagate,

and since the Virus originated from TV1 and this from broken up and metallic treated cells –

TV! WAS REPORTED - - - - - PROPAGATING VIRUS PRESENT

ALIQUOT 2 (CELLS DESTROYED) WAS REPORTED – VIRUS PRESENT

- - - - - - - - - - -

THE ANTI-VIRUS AGENT UNDER TEST WAS – POLYMER OCT 57

F. Biostatic test

½ gr. Of antistatic agent was mixed with Aliquot 5 (Aliquot 5 having been shown to be a rapidly growing culture as was the control aliquot, Aliquot 6) and the

 

 

 

77

 

 

 

 

 

mixture held at 26-30 C. for 10 and 48 hours. When examination of Aliquot 6 compared to Aliquot 5 containing the Antiviral agent was made,

Aliquot 6 was a normally growing culture – microscopic examination revealed normally dividing mobile cells.

Aliquot 5 plus the test Antiviral agent was a normally growing culture – microscopic examination revealed normally dividing mobile cells indistinguishable from the control culture.

Since no deleterious effect was noted from the presence of the antiviral agent-

POLYMER OCT 57 WAS REPORTED - - - - NON-BIOSTATIC

G. ANTI VIRUS EVALUATION OF THE POLYMER

SINCE Aliquot 3 milled with and divided into part Aliquot 3 TV1 and Aliquot 3 TV2 was shown under "D" above that Virus/Zipoid was present, Aliquot 3 TV2 was mixed with ½ gr. of Anti virus test polymer OCT 57

(This is experimentally equivalent to putting the Anti viral polymer into the extracellular medium of a virus infected bioform).

The mixture was held at 26-30 C. for 10 minutes and mixed with the rapidly growing normal culture Aliquot 4.

(this is experimentally equivalent to putting the extracellular fluid containing the virus and the antiviral agent into contact with the normally growing

 

 

 

78

 

 

cells of an organism)

Aliquot 4 now contained –

normal cells in the cultures under study

known virus destructive to normal culture

Antiviral agent OCT 57 known to be

NON-BIOSTATIC

Aliquot 4 was examined grossly and microscopically compared to the normal control Aliquot 6.

 

Aliquot 4 Aliquot 6

with Antiviral no treatment, control

agent OCT 57

with actively

destructive virus TV2

- - - - - examination after 10 hours - - - - - - -

normal cells normal cells

microscopically normal microscopically normal

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Since polymer OCT 57 was shown by the above to render inactive the virus, shown to be previous to treatment very active in "E" above specifically a portion of Aliquot 2 TV2,

POLYMER OCT 57 WAS REPORTED - - - ANTIVIRUS ACTIVITY PRESENT

 

 

 

 

 

 

79

 

 

 

 

 

 

 

Tests of Polymer OCT 57 on naturally occurring

bacteriophage to B. Coli bacteria.

Ten aliquots of 100 ml. each were made of an actively growing culture of B. Coli in beef broth. The cultures were turbid due to bacterial growth grossly observed as well as microscopically.

Bacteriophage to B. Coli was detected and acquired by adding 25 ml. of surface sewage water obtained from different sewage locales to culture aliquots 1, 2, 3, and 4.

Aliquots 2 and 3 cleared within 4 hours after addition of the sewage water. Aliquot 3 was selected as the active bacteriophage-virus.

The bacteriophage transmission effect was further evaluated by adding ¼ of aliquot 3 to each of aliquot 4 and 5. These aliquots cleared of B. Coli within 3 hours (this showed aliquot 3 contained a transmittable bacteriophage-virus). To the remaining ½ of aliquot 3 (shown to contain bacteriophage-virus) 0.4 grams of OCT 57 was added. After 5 hours, this virus-containing-polymer was divided and added to each of aliquots 7 and 8. During the next 24 hours there was no decrease in growth of the contained B. Coli. These cultures were tested for freedom from bacteriophage by addition to aliquots 9 and 10. Since active growth occurred in these aliquots it is clear that the Polymer OCT 57 stopped the action of the bacteriophage to B. Coli.

 

 

 

 

 

 

 

 

80

 

 

 

 

 

 

 

 

 

 

Polymer OCT 57 Test Series AMLI

Tests on an active case of AIDS.

- - - - - - - - - - - - - - - - - - - - - - - - -

A human male was selected (ML1) as the AIDS case. He was reported by numerous tests to carry an active case of AIDS. Estimated acquisition of AIDS two years before start of test. The human male demonstrated the signs of an active case of AIDS at the start of the test.

A control normal CT known to be free of AIDS was selected. To the control normal no treatment by Polymer OCT 57 or any other treatment was applied.

To the active AIDS male ML1 approximately one gram of Polymer OCT 57 was applied as a salve to the groin of the male. Repetition of the application of one gram of Polymer OCT 57 was made once each week for a test period of eight weeks. No other treatment was given to the male during the eight week period. After the elapse of eight weeks the male ML1 was again examined for signs of AIDS.

 

 

 

 

 

 

 

 

81

 

 

 

After the test period compared to the starting condition and the normal CT individual test results were:

- - - - - - - - - - - - - - - - - - - - -

Test AIDS male AIDS male after Control

at start 8 weeks of Aids free

OCT 57 treatment individual

Hemoglobin

100% is norm. 82 94 98

red cell

poikilocytosis 3% 0 0

PMN polymorpho-

nuclear

neutrophilic/cmn 4,200 8,100 9,200

monocytes

% to PMN 0 5 3

eosinophiles

% to PMN 5 1 0

basophiles

% to PMN 3 1 0

AIDS virus

protein test POS NEG NEG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

 

 

82

 

 

EXAMPLE 2

X - X

X - X

X - X

X - X

X - X

TESTS ON THE PRODUCT-

BIOSTATIC EFFECT NONE TO YEAST

BIOSTATIC EFFECT NONE TO B. COLI

ANTI VIRAL ACTIVITY MINIVERTREBRATE VIRUS/ EFFECTIVE

ANTI VIRAL ACTIVITY B. COLI BACTERIOPHAGE/

EFFECTIVE

 

- - - - - - - - - - - - - - - - - - - - - - -

EXAMPLE 3

X - X

X - X

X - X

X - X

X - X

TESTS ON THE PRODUCT-

BIOSTATIC EFFECT NONE TO YEAST

BIOSTATIC EFFECT NONE TO B. COLI

ANTI VIRAL ACTIVITY MINIVERTREBRATE VIRUS/ EFFECTIVE

ANTI VIRAL ACTIVITY B. COLI BACTERIOPHAGE/

EFFECTIVE

 

 

 

 

 

83

 

 

 

 

EXAMPLE 4

X - X

X - X

X - X

X - X

X - X

TESTS ON THE PRODUCT-

BIOSTATIC EFFECT NON BIOSTATIC/ YEAST

BIOSTATIC EFFECT NON BIOSTATIC/ B. COLI

ANTI VIRAL ACTIVITY PRESENT TO YEAST

ANTI VIRAL ACTIVITY PRESENT TO BACTERIOPHAGE OF B.COLI

- - - - - - - - - - - - - - - - - - -

 

EXAMPLE 5

X - X

X - X

X - X

X - X

X - X

TESTS ON THE PRODUCT-

BIOSTATIC EFFECT NO EFFECT TO YEAST

BIOSTATIC EFFECT NO EFFECT TO B. COLI

ANTI VIRAL ACTIVITY DESTROYS YEAST VIRUS

ANTI VIRAL ACTIVITY DESTROYS MINIVERTREBRATE VIRUS

 

 

 

 

 

84

 

 

 

 

EXAMPLE 6

X - X

X - X

X - X

X - X

X - X

TESTS ON THE PRODUCT-

BIOSTATIC EFFECT NO IRRITATION TO HUMAN

BIOSTATIC EFFECT NO IRRITATION TO SMALL VERTEBRATE

ANTI VIRAL ACTIVITY EFFECTIVE AGAINST SALMONELLA BACTERIOPHAGE

ANTI VIRAL ACTIVITY EFFECTIVE AGAINST B. COLI BACTERIOPHAGE

 

 

 

 

EXAMPLE 7

X - X

X - X

X - X

X - X

X - X

TESTS ON THE PRODUCT-

BIOSTATIC EFFECT NONE TO YEAST

BIOSTATIC EFFECT NON IRRITATING TO HUMAN

ANTI VIRAL ACTIVITY EFFECTIVE AGAINST YEAST VIRUS

ANTI VIRAL ACTIVITY EFFECTIVE AGAINST HUMAN AIDS

 

 

 

85

 

 

EXAMPLE 8

X - X

X - X

X - X

X - X

X - X

TESTS ON THE PRODUCT-

BIOSTATIC EFFECT NONE TO B. COLI

BIOSTATIC EFFECT NONE TO YEAST

ANTI VIRAL ACTIVITY EFFECTIVE AGAINST B. COLI BACTERIOPHAGE

 

 

 

 

 

 

EXAMPLE 9

X - X

X - X

X - X

X - X

X - X

TESTS ON THE PRODUCT-

BIOSTATIC EFFECT NONE TO B. COLI

BIOSTATIC EFFECT NO IRRITATION TO TOBACCO PLANT

ANTI VIRAL ACTIVITY EFFECTIVE TO YEAST

ANTI VIRAL ACTIVITY EFFECTIVE TO TOBACCO VIRUS

 

 

 

 

 

 

86

 

 

 

 

 

 

 

 

Example 10.

A polymer was prepared by heating together with agitation

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX until the

evolution of water stopped then adding XXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXX was reached.

A polymer of XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXX resulted.

Tested on an individual with Herpes inflammatory vescule

disease resulted in a clearing of the adverse symptoms.

Tested on an individual with Monilia Candidasis resulted in promptclearing of the symptoms of the infestation and no recurrence after 8 months.

Toxicity tests to mammals showed this product of this example to be non-toxic.

 

 

 

 

 

 

 

 

 

87

 

 

 

 

CLAIM 1

A polyester polymer

of sufficient length to prevent its penetrating into a cell,

terminated on each end with a carboxy replofile.

 

 

CLAIM 2

A process for treating virus infestation in a mammal which comprises administering to the mammal an amount of pharmaceutical composition

effective to provide

destruction of the virus

said pharmaceutical composition

comprising

a polyester polymer

of sufficient chain length

to prevent its penetrating into a cell

said polyester polymer

terminated on both ends with a carboxy

replofile.

 

 

 

 

 

88

 

 

 

CLAIM 3

A polyester polymer

of chain length of between XXX

and XXX repeating units, said

polyester,

terminated on each end with a

XXXXX XXXXX XXXXXXXXX.

 

 

CLAIM 4

A process for treating virus infestation in an aqueous

biological cell containing medium which comprises administering to the medium an amount of pharmaceutical composition

effective to provide

destruction of the virus

said pharmaceutical composition comprising

a polyester polymer

of chain length between XXX and

XXX repeating units

said polyester polymer

terminated on both ends with

a XXXXX XXXXX XXXXXXXXX.

 

 

 

 

89

 

 

CLAIM 5

A polyester polymer

of chain length of XXX repeating units of XXXXX XXXXXXXXXXX, XX of said units having a side group of XXXXXXXX XXXXXXXX residues, the polymer

terminated on each end with

XXXXXXX XXXXXXXXXXX.

 

CLAIM 6

A process for treating human AIDS virus infestation

in a human being

which comprises administering

to the human an amount of

pharmaceutical composition

effective to provide

destruction of the AIDS virus

said pharmaceutical composition

comprising

a polyester polymer

of chain length of XXX repeating units of XXXXXX XXXXX XXX, XX of which have side chains of XXXX XXXXXXXX XXXXX residues

said polyester polymer,

terminated on both ends with

XXXXX XXXXXXXX XXXXXX.

 

 

 

90

 

 

 

 

CLAIM 7

A polyester polymer

of chain length of XXX repeating units of XXXXX XXXXXXXXXXX, the polymer

terminated on each end with

XXXXXXX XXXXXXXXXXX.

 

CLAIM 8

A process for treating human AIDS virus infestation

in a human being

which comprises administering

to the human an amount of

pharmaceutical composition

effective to provide

destruction of the AIDS virus

said pharmaceutical composition

comprising

a polyester polymer

of chain length of XXX repeating units of XXXXXX XXXXX XXX

said polyester polymer,

terminated on both ends with

XXXXX XXXXXXXX XXXXXX.

 

 

 

 

 

91

 

 

 

 

 

CLAIM 9

A polyester polymer

of chain length of XXX repeating units of XXXXX XXXXXXXXXXX, the polymer

terminated on each end with

XXXXXXX XXXXXXXXXXX.

 

CLAIM 10

A process for treating human AIDS virus infestation

in a human being

which comprises administering

to the human an amount of

pharmaceutical composition

effective to provide

destruction of the AIDS virus

said pharmaceutical composition

comprising

a polyester polymer

of chain length of XXX repeating units of XXXXXX XXXXX XXX

said polyester polymer,

terminated on both ends with

XXXXX XXXXXXXX XXXXXX.

 

 

 

 

92

 

 

 

CLAIM 11

A polyester polymer

of chain length of XXX repeating units of XXXXX XXXXXXXXXXX, the polymer

terminated on each end with

XXXXXXX XXXXXXXXXXX.

 

CLAIM 12

A process for treating tobacco plant virus infestation

in a tobacco plant

which comprises administering

to the human an amount of

pharmaceutical composition

effective to provide

destruction of the AIDS virus

said pharmaceutical composition

comprising

a polyester polymer

of chain length of XXX repeating units of XXXXXX XXXXX XXX

said polyester polymer,

terminated on both ends with

XXXXX XXXXXXXX XXXXXX.

 

 

 

 

 

 

93

 

 

 

Comment on the origin of the survey THE AIDS VIRUS / Howard C. Woodruff 1988-

The text of the survey "THE AIDS VIRUS" is in fact the Specification of a Patent Application sent to the United States Patent Office on March 18, 1988 bearing the title POLYMERS FOR DESTROYING VIRUS. The U.S. Patent Office acknowledged receival of it as of March 21, 1988.

The documents relative to the Patent Application are reproduced herein on pages:

95 Request for Patent filing to the Commissioner of Patents

96 Request to make special due to age of applicant

97 Oath that applicant is the sole inventor

98 Small Entity Declaration that the inventor is independent

99 Petition to the Commissioner of Patents for a patent the Specification of which follows (and is herein covered in THE AIDS VIRUS pages 1 to 93.

- - - - - - - - - - - - - - - - - - - - - - - - -

Thus the survey - THE AIDS VIRUS contains all the data and documents in connection with the U.S. Patent application "Polymers for destroying Virus".

Serial Number 07/170 913

In Patent Office 03/21/88

 

 

 

 

 

 

 

94

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Woodruff -I- -II- -III- HOMEH.C. WOODRUFF 1988 - Pocket Theory Publishing 2004 - MALLEUS.NET